世博娱乐

2004.08-2009.09     纽约大学，化学，博士，导师: Ned Seeman院士

1999.09-2004.07     中国科学技术大学，应用化学，学士，导师: 钱逸泰院士

2022.01-                  上海交通大学-世博娱乐 /转化医学院，研究员

2015.06-2021.12     复旦大学-生物医学研究院，研究员

2009.11-2015.05     耶鲁大学，分子生物学，博士后，导师: Ronald Breaker院士

擅长核酸编辑和组装，致力于开拓DNA在纳米材料、生物传感、临床诊断等方面的基础和应用研究，开展的方向包括：

• 对靶点或标志物明确的重大恶性肿瘤和慢性疾病，开发可用于体液诊断的便捷适配体探针（试剂盒）；对于标志物未知的重大恶性肿瘤和慢性疾病（主要是三阴性乳腺癌），以适配体探针库为抓手，通过临床样本间的对冲抵消，筛选鉴定疾病相关的标志物和对应的检测探针，揭示疾病的分子机制并明确药物可作用的靶点；

• 开发新型3D框架核酸的设计和组装方案，在保持组装体近原子分辨率的前提下，将自组装的尺度在3D空间从纳米推向微米和亚毫米级，以满足其作为介质在材料科学中的更多应用需求；同时开发活细胞生产制备框架核酸的生物技术，降低制备成本，为框架核酸的临床研究提供支持；

• 利用试管内进化的高效筛选策略分离获得重大疾病相关的核酸酶（DNAzyme和Ribozyme），将其与框架核酸融合，编辑组装“环境响应型智能纳米药物载体”，用于重大疾病的靶向精确递药。
  

• 国自然基金委创新研究群体 “肿瘤免疫治疗新策略”

      项目编号：82121002，  2022-2026  骨干 主持  200万

• 科技部重点研发计划 “微纳生物机器人的定向合成和诊疗应用”（课题一）

      课题编号：2020YFA0908901，  2020-2025  主持  604万

• 国自然基金委重大研究计划 “肿瘤演进与诊疗的分子功能可视化研究”（培育项目）

      项目编号：91859104，  2019-2021  主持  60万

• 江苏贝格尔生物医药有限公司 横向合作课题“外泌体捕捉与生物标记物检测开发”

      2019-2020  主持  30万

• 国自然国际（地区）合作与交流重大项目“靶向治疗类风湿性关节炎的核酸纳米抗体研究”

      项目编号：81861138004，  2018-2020  共同主持  总经费300万，个人承担150万

• 国自然面上项目 “DNA纳米骨架的组装及其在生物和材料学中的应用”

      项目编号：21673050，  2017-2020  主持  已结题  68万

• 科技部国家重点研发计划“重大慢性非传染性疾病防控研究”

       项目编号：2016YFC1306400， 2016-2020  子课题负责人  45.4万

• 主持国家级青年人才项目   2015-2018  主持  300万

代表性文章（独立PI期间）

• Qinqin Hu^¥; Zongxuan Tong^¥; Ayimukedisi Yalikong^¥; Li-Ping Ge^¥; Qiang Shi; Xinyu Du; Pu Wang; Xi-Yu Liu; Wuqiang Zhan; Xia Gao; Di Sun; Tong Fu; Dan Ye; Chunhai Fan; Jie Liu; Yun-Shi Zhong*; Yi-Zhou Jiang*; Hongzhou Gu*. “DNAzyme-Based Faithful Probing and Pulldown to Identify Candidate Biomarkers of Low Abundance.” Nature Chemistry 2024, 16, 122-131.

• Mo Zhou^¥; Tianbin Xu^¥; Kai Xia; Haiqing Gao; Wei Li; Tingting Zhai; Hongzhou Gu*. “Small DNAs That Specifically and Tightly Bind Transition Metal Ions.” Journal of the American Chemical Society 2023, 145, 16, 8776-8780.

• Xiao Chu; Xinyu Du; Longhua Yang; Ziyi Wang; Yi Zhang; Xiaonan Wang; Lijun Dai; Jiangnan Zhang; Jie Liu; Nan Zhang*; Yongxing Zhao*; Hongzhou Gu*. “Targeting Tumor Necrosis Factor Receptor 1 with Selected Aptamers for Anti-Inflammatory Activity.” ACS Applied Materials & Interfaces 2023, 15, 11599-11608.

• Qiao Zhang^¥; Kai Xia^¥; Meng Jiang^¥; Qingting Li; Weigang Chen; Mingzhe Han; Wei Li; Rongqin Ke; Fei Wang; Yongxing Zhao; Yuehua Liu; Chunhai Fan; Hongzhou Gu*. “Catalytic DNA-Assisted Mass Production of Arbitrary Single-Stranded DNA.” Angewandte Chemie International Edition 2023, e202212011.

• Jin Liu^¥; Liman Chen^¥; Tingting Zhai; Wei Li; Yuehua Liu; Hongzhou Gu*. “Programmable Assembly of Amphiphilic DNA through Controlled Cholesterol Stacking.” Journal of the American Chemical Society 2022, 144, 36, 16598-16603.

• Yang Yang^¥; Zhenyong Wu^¥; Laurie Wang; Kaifeng Zhou; Kai Xia; Qiancheng Xiong; Longfei Liu; Zhao Zhang; Edwin R Chapman; Yong  Xiong; Thomas J Melia; Erdem Karatekin; Hongzhou Gu*; Chenxiang Lin*. “Sorting liposomes of distinct sizes by DNA-brick assisted centrifugation.” Nature Chemistry 2021, 13, 335-342.

• Youli Jia^¥; Liman Chen^¥; Jin Liu; Wei Li; Hongzhou Gu*. “DNA Catalyzed Efficient Production of Single-stranded DNA Nanostructures.” Chem 2021, 7, 4, 959-981.

• Qingting Li; Zongxuan Tong; Yichun Cao; Hongzhou Gu*. “DNAs catalyzing DNA nanoconstruction.” Chem 2021, 7, 10, 2556-2568.

• Canyu Zhang; Qingting Li; Tianbin Xu; Wei Li; Yungang He; Hongzhou Gu*. “New DNA-hydrolyzing DNAs isolated from an ssDNA library carrying a terminal hybridization stem.” Nucleic Acids Research 2021, 49, 11, 6364-6374.

• Xinyu Du^¥; Xiaoqin Cheng^¥; Wei Li; Zhilei Ge; Chunjiu Zhong; Chunhai Fan; Hongzhou Gu*. "Engineering allosteric ribozymes to detect thiamine pyrophosphate in whole blood.” Analytical Chemistry 2021, 93, 9, 4277-4284.

• Tianbin Xu^¥; Canyu Zhang^¥; Kai Xia; Wei Li; Yichun Cao; Hongzhou Gu*. “Small DNAs that bind nickel(II) specifically and tightly.” Analytical Chemistry 2021, 93, 45, 14912-14917.

• Qingting Li; Shu Zhang; Wei Li; Zhilei Ge; Chunhai Fan; Hongzhou Gu*. “Programming CircLigase catalysis for DNA rings and topologies.” Analytical Chemistry 2021, 93, 3, 1801-1810.

• Jin Liu; Hongzhou Gu*. “Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes.” STAR Protocols 2021, 2, 2, 100531.

• Huiyan Li; Fangfei Yin; Xinyi Li; Wannan Jia; Jia Ding; Li Zhang; Zhonghua Wang; Qinqin Hu; Jieliang Zuo; Huliang Jia; Lihua Wang; Chunhai Fan*; Hongzhou Gu*; Jian Wu*. “Novel aptasensor-based assay of sonic hedgehog ligand for detection of portal vein invasion of hepatocellular carcinoma.” Biosensors and Bioelectronics 2021, 174, 112738.

• Dong-Jie Tang^¥; Xinyu Du^¥; Qiang Shi^¥; Jian-Ling Zhang^¥; Yuan-Ping He; Yan-Miao Chen; Zhenhua Ming; Dan Wang; Wan-Ying Zhong; Yu-Wei Liang; Jin-Yang Liu; Jian-Ming Huang; Yun-Shi Zhong; Hongzhou Gu*; Ji-Liang Tang*. “A SAM-I riboswitch with the ability to  sense and respond to uncharged initiator tRNA.” Nature Communications 2020, 11, 2794, 1-11.

• Kai Xia; Jianlei Shen; Qian Li; Chunhai Fan*; Hongzhou Gu*. “Near-Atomic Fabrication with Nucleic Acids.” ACS Nano 2020, 14, 1319-1337.

• Ziyi Wang; Xiao Chu; Na Li; Lingling Fu; Hongzhou Gu*; Nan Zhang*. “Engineered DNA nanodrugs alleviate inflammation in inflammatory arthritis.” International Journal of Pharmaceutics 2020, 577, 119047.

• Zongxuan Tong; Qinqin Hu; Hongzhou Gu*. “Deoxyribozymes: selection, biosensing and outlook.” Chemical Journal of Chinese Universities 2020, 41(11), 2345-2355.

• Qinqin Hu^¥; Hua Li^¥; Lihua Wang; Hongzhou Gu*; Chunhai Fan*. “DNA Nanotechnology Enabled Drug Delivery systems.” Chemical  Reviews 2019, 119, 6459-6506.

• Hua Li; Jin Liu; Hongzhou Gu*. “Targeting Nucleolin to Obstruct Vasculature Feeding with An Intelligent DNA Nanorobot.” Journal of Cellular and Molecular Medicine 2019, 23, 2248-2250.

• Xinyu Du^¥; Xin Zhong^¥; Wei Li; Hua Li*; Hongzhou Gu*. “Retraining and Optimizing DNA-Hydrolyzing Deoxyribozymes for Robust Single- and Multiple-Turnover Activities.” ACS Catalysis 2018, 8, 5996-6005.

• Zhilei Ge^¥; Hongzhou Gu^¥; Qian Li; Chunhai Fan*. “Concept and Development of Framework Nucleic Acids.” Journal of the American Chemical Society 2018, 140, 51, 17808-17819.

• Qinqin Hu; Sheng Wang; Lihua Wang; Hongzhou Gu*; Chunhai Fan*. “DNA Nanostructure-Based Systems for Intelligent Delivery of Therapeutic Oligonucleotides.” Advanced Healthcare Materials 2018, 7, 1701153.

• Hongzhou Gu*. “Single-molecule Genotyping with Nanoscale DNA Shape IDs.” Science China Chemistry 2017, 60, 1111-1112.

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代表性文章（博士-博后期间）

• Hongzhou Gu; Kazuhiro Furukawa; Zasha Weinberg; Daniel F. Berenson; Ronald R. Breaker. “Small, Highly-active DNAs that Hydrolyze DNA.” Journal of the American Chemical Society 2013, 135, 9121-9129.

• Hongzhou Gu; Ronald R. Breaker. “Production of Single-stranded DNAs by Self-cleavage of Rolling-circle Amplification Products.” BioTechniques 2013, 54, 337-343.

• Hongzhou Gu; Kazuhiro Furukawa; Ronald R. Breaker. “Engineered Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP.” Analytical Chemistry 2012, 84, 4935-4941.

• Hongzhou Gu; Jie Chao; Shou-Jun Xiao; Nadrian C. Seeman. “A Proximity-Based Programmable DNA Nanoscale Assembly Line.” Nature 2010, 465, 202-205.

• Hongzhou Gu; Wei Yang; Nadrian C. Seeman. “DNA Scissors Device Used to Measure MutS Binding to DNA Mis-Pairs.” Journal of the American Chemical Society 2010, 132, 4352-4357.

• Hongzhou Gu; Jie Chao; Shou-Jun Xiao; Nadrian C. Seeman. “Dynamic Patterning Programmed by DNA Tiles Captured on a DNA Origami Substrate.” Nature Nanotechnology 2009, 4, 245-248.

已建成“核酸生物医学工程与技术”平台，并形成了包括1名助理研究员、3名博士后、12名博士生、3名科研助理的研究团队，成员多次获得“上海市超级博士后激励计划”、“博士生国家奖学金”、“上海市优秀毕业生”等奖励和称号。

主要专利

•  一种检测硫胺素焦磷酸的RNA传感器及其应用

       专利号：ZL201810077122.9

       发明人：顾宏周、杜鑫雨、钟春玖、程小芹

•  能快速水解DNA的脱氧核酶突变体

       专利号：ZL201810131100.6

       发明人：顾宏周

•  一种脱氧核酶切割方法

       专利号：ZL201810131087.4

        发明人：顾宏周

•  提高单链DNA连接效率的方法

       专利号：ZL202011011345.9

       发明人：顾宏周、李青婷

•  一种长单链DNA的制备方法

       专利号：201910989379.6

       发明人：顾宏周、张俏

• 能快速水解DNA的II类脱氧核酶突变体

       专利号：201910989374.3

       发明人：顾宏周、张俏

• 一种富集、鉴定待测病样中分子标志物的方法

       专利号：202110361337.5

       发明人：顾宏周、胡沁沁

• 差异筛选特异感应三阴性乳腺癌的脱氧核酶探针

       专利号：202110364792.0

       发明人：顾宏周、胡沁沁

• 一种利用单链DNA纳米结构辅助分选脂质体的方法

       专利号：202010773498.0

       发明人：顾宏周、陈黎曼

• III类脱氧核酶突变体及其制备方法与应用

       专利号：202011120065.1

       发明人：顾宏周、张灿钰、李青婷

• IV类脱氧核酶突变体及其制备方法与应用

      专利号：202011118638.7

      发明人：顾宏周、张灿钰、李青婷

• DNAzyme and use thereof

      专利号：PCT/CN2022/085696

       发明人：顾宏周、张俏、夏凯、李富友

       申请人：Fudan University；Shanghai Wuti Biotechnology Co., Ltd.

 个人奖项

• 2014年获评国家级青年人才

• 2017年上海市高层次人才项目

• 2022年上海市优秀学术带头人
  

长期招收博士生、博士后及助理研究员！